Comparative characterization of the cellular uptake and photodynamic efficiency of Foscan® and Fospeg in a human prostate cancer cell line

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dc.contributor.author Petri, A en
dc.contributor.author Yova, D en
dc.contributor.author Alexandratou, E en
dc.contributor.author Kyriazi, M en
dc.contributor.author Rallis, M en
dc.date.accessioned 2014-03-01T11:46:40Z
dc.date.available 2014-03-01T11:46:40Z
dc.date.issued 2012 en
dc.identifier.issn 15721000 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/38013
dc.subject Foscan en
dc.subject Fospeg en
dc.subject Photodynamic therapy en
dc.subject Prostate cancer cells en
dc.title Comparative characterization of the cellular uptake and photodynamic efficiency of Foscan® and Fospeg in a human prostate cancer cell line en
heal.type other en
heal.identifier.primary 10.1016/j.pdpdt.2012.03.008 en
heal.identifier.secondary http://dx.doi.org/10.1016/j.pdpdt.2012.03.008 en
heal.publicationDate 2012 en
heal.abstract Background: m-THPC (Foscan®) is one of the most potent second generation photosensitizers used in photodynamic therapy, photoactivated at higher wavelengths (652 nm). However, its strongly hydrophobic nature causes aggregation of the molecules and prevents its unbiased bioavailability in the biological media, resulting in lower accumulation in the tumor cells. Several strategies have been adopted to improve the photodynamic characteristics of the photosensitizer. Among them, very promising seems to be the encapsulation of the molecule into liposomes, due to the superior properties of liposomes as drug carriers. Methods: In this paper the photodynamic characteristics of the PEGylated liposomal formulation of m-THPC, Fospeg, using the human prostate cancer cell line LNCaP, as an in vitro model, were investigated. In addition the spectral characteristics, cellular uptake and localization, dark and light induced cytotoxicity and photodynamic efficacy of Foscan® and Fospeg were compared. Results: Fospeg, compared with Foscan, showed higher intracellular uptake at any concentration and incubation time. Regarding PDT efficacy, Fospeg produced more severe cytotoxicity than Foscan® at any concentration and energy dose. Using Fospeg, the lowest concentration (0.22 μM) and energy dose (180 mJ/cm2) was adequate to result in the death of 50% of the cells 24 h post PDT while an approximately 10 times higher Foscan® concentration (1.8 μM) was needed to result in the same cytotoxicity. Conclusions: The use of the PEGylated liposomal formulation of m-THPC resulted in the improvement of its intracellular uptake and the enhancement of its photodynamic activity. Fospeg, compared to Foscan®, proved to be a more advantageous photosensitizer for photodynamic therapy. © 2012 Elsevier B.V. All rights reserved. en
heal.journalName Photodiagnosis and Photodynamic Therapy en
dc.identifier.doi 10.1016/j.pdpdt.2012.03.008 en

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