Evaluation of Anti-oxidant Activity and Identification of Major Polyphenolics of the Invasive Weed Oxalis pes-caprae

Gucluturk, I; Detsi, A; Weiss, EK; Ioannou, E; Roussis, V; Kefalas, P

dc.contributor.author	Gucluturk, I	en
dc.contributor.author	Detsi, A	en
dc.contributor.author	Weiss, EK	en
dc.contributor.author	Ioannou, E	en
dc.contributor.author	Roussis, V	en
dc.contributor.author	Kefalas, P	en
dc.date.accessioned	2014-03-01T11:46:59Z
dc.date.available	2014-03-01T11:46:59Z
dc.date.issued	2012	en
dc.identifier.issn	09580344	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/38042
dc.subject	Anti-oxidant activity	en
dc.subject	Cernuoside	en
dc.subject	Oxalis pes-caprae	en
dc.subject	Polyphenols	en
dc.subject	Weeds	en
dc.title	Evaluation of Anti-oxidant Activity and Identification of Major Polyphenolics of the Invasive Weed Oxalis pes-caprae	en
heal.type	other	en
heal.identifier.primary	10.1002/pca.2367	en
heal.identifier.secondary	http://dx.doi.org/10.1002/pca.2367	en
heal.publicationDate	2012	en
heal.abstract	Introduction: Phytochemical analyses of weeds, many of which have been used in traditional medicine worldwide, could lead to the identification of secondary metabolites with significant biological activity. Objective: To perform an assessment of the chemical composition and exploitation potential of the invasive weed Oxalis pes-caprae. To evaluate the anti-oxidant activity of its extracts and isolate and characterise polyphenolic metabolites using LC-DAD-MS (ESI+) and NMR methods. Methodology: Aerial parts of the invasive weed O. pes-caprae were extracted with solvents of increasing polarity and their major polyphenolic metabolites were identified by LC-DAD-MS (ESI+). The total phenolic content of the extracts was determined using the Folin-Ciocalteu method, while their anti-oxidant activity was evaluated on the basis of their ability to scavenge the stable free radical 1,1-diphenyl-2-picrylhydrazyl and hydrogen peroxide. Results: The major polyphenolic constituents of the extracts were tentatively characterised as chlorogenic acid, quinic ferulate, luteolin glucoside and cernuoside according to their MS and UV spectroscopic data. Cernuoside, an aureusidin glucoside, was isolated from the methanolic extract of the weed's flowers and its structure was unambiguously identified by 1D- and 2D-NMR spectroscopy. The butanol extract of O. pes-caprae displayed the highest anti-oxidant activity. Conclusion: The metabolic profile of O. pes-caprae was studied and the structures of the major polyphenolic metabolites based on their MS and UV-vis spectra were tentatively assigned. The aureusidin glucoside cernuoside was isolated and characterised for the first time from O. pes-caprae. The extracts exhibited high levels of anti-oxidant activity. © 2012 John Wiley & Sons, Ltd.	en
heal.journalName	Phytochemical Analysis	en
dc.identifier.doi	10.1002/pca.2367	en