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Evaluation of Anti-oxidant Activity and Identification of Major Polyphenolics of the Invasive Weed Oxalis pes-caprae

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dc.contributor.author Gucluturk, I en
dc.contributor.author Detsi, A en
dc.contributor.author Weiss, EK en
dc.contributor.author Ioannou, E en
dc.contributor.author Roussis, V en
dc.contributor.author Kefalas, P en
dc.date.accessioned 2014-03-01T11:46:59Z
dc.date.available 2014-03-01T11:46:59Z
dc.date.issued 2012 en
dc.identifier.issn 09580344 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/38042
dc.subject Anti-oxidant activity en
dc.subject Cernuoside en
dc.subject Oxalis pes-caprae en
dc.subject Polyphenols en
dc.subject Weeds en
dc.title Evaluation of Anti-oxidant Activity and Identification of Major Polyphenolics of the Invasive Weed Oxalis pes-caprae en
heal.type other en
heal.identifier.primary 10.1002/pca.2367 en
heal.identifier.secondary http://dx.doi.org/10.1002/pca.2367 en
heal.publicationDate 2012 en
heal.abstract Introduction: Phytochemical analyses of weeds, many of which have been used in traditional medicine worldwide, could lead to the identification of secondary metabolites with significant biological activity. Objective: To perform an assessment of the chemical composition and exploitation potential of the invasive weed Oxalis pes-caprae. To evaluate the anti-oxidant activity of its extracts and isolate and characterise polyphenolic metabolites using LC-DAD-MS (ESI+) and NMR methods. Methodology: Aerial parts of the invasive weed O. pes-caprae were extracted with solvents of increasing polarity and their major polyphenolic metabolites were identified by LC-DAD-MS (ESI+). The total phenolic content of the extracts was determined using the Folin-Ciocalteu method, while their anti-oxidant activity was evaluated on the basis of their ability to scavenge the stable free radical 1,1-diphenyl-2-picrylhydrazyl and hydrogen peroxide. Results: The major polyphenolic constituents of the extracts were tentatively characterised as chlorogenic acid, quinic ferulate, luteolin glucoside and cernuoside according to their MS and UV spectroscopic data. Cernuoside, an aureusidin glucoside, was isolated from the methanolic extract of the weed's flowers and its structure was unambiguously identified by 1D- and 2D-NMR spectroscopy. The butanol extract of O. pes-caprae displayed the highest anti-oxidant activity. Conclusion: The metabolic profile of O. pes-caprae was studied and the structures of the major polyphenolic metabolites based on their MS and UV-vis spectra were tentatively assigned. The aureusidin glucoside cernuoside was isolated and characterised for the first time from O. pes-caprae. The extracts exhibited high levels of anti-oxidant activity. © 2012 John Wiley & Sons, Ltd. en
heal.journalName Phytochemical Analysis en
dc.identifier.doi 10.1002/pca.2367 en


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